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    E-ISSN: 2149-4533 ISSN: 1300-865X
    Comparison of Diatoms Which were Obtained from The External Surface of The Body and Internal Organs in the Corpses Pulled Out of Water Using Colloidal Silica Gradient Centrifuge Method
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    Research Article
    VOLUME: 24 ISSUE: 2
    P: 83-92
    August 2019

    Comparison of Diatoms Which were Obtained from The External Surface of The Body and Internal Organs in the Corpses Pulled Out of Water Using Colloidal Silica Gradient Centrifuge Method

    The Bulletin of Legal Medicine 2019;24(2):83-92
    DOI: 10.17986/blm.2019252241
    Çiler Fulya Erkan
    Gürsel Çetin
    1. İstanbul Üniversitesi-Cerrahpaşa, Adli Tıp Enstitüsü, Fen Bilimleri Anabilim Dalı, İstanbul
    2. İstanbul Üniversitesi-Cerrahpaşa, Cerrahpaşa Tıp Fakültesi, Adli Tıp Anabilim Dalı, İstanbul
    No information available.
    No information available
    Received Date: 14.06.2018
    Accepted Date: 09.05.2019
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    ABSTRACT

    Objective:

    Diatom analysis is a valuable tool in forensic science, and it is useful in the diagnosis of drowning and determination of the drowning site. The basic principle of the “diatom test” in investigation of drowning is based on correlation between diatoms are present in the medium where the possible drowning took place and inhalation of water causes penetration of diatoms into the alveolar system and bloodstream and consequently their deposition into brain, kidneys and other organs, like the bone marrow of large bones. There are various extraction methods that are used to isolate diatoms from water and tissues. Nitric acid digestion is a worldwide known method for the extraction of diatoms. In this study, it was aimed to obtain diatom by using colloidal silica centrifuge method instead of acid method besides it was aimed to compare diatom obtained from the external surfaces of body and diatom obtained from the internal organs.

    Materials and Methods:

    Therefore, internal organ and body fluid samples that have been obtained from 30 corpses which were removed from the water and brought to the Council of Forensic Medicine to perform autopsy, were examined and diatom were obtained from samples of 19 cases. Moreover, the diatoms that were obtained from the swab samples taken from the outer body surfaces and the diatoms obtained from the internal organs were compared.

    Results:

    When the diatoms which were obtained from tissues from internal organs and body fluids were evaluated numerically, it was seen that the diatoms that were obtained lungs were in high numbers and it was followed by the pleural liquid, stomach content and pericardial fluid, respectively. The method, which is used, it was possible to obtain plankton other than diatom from the tissues.

    Conclusion:

    According to the findings which were obtained swap samples, more diatoms were obtained from the nasal swap than the other swap samples. Colloidal Silica Gradient Centrifugation Method is more advantageous than traditional Nitric Acid Digestion Method because of that it is nontoxic, reliable, easy to apply and detecting plankton other than diatoms.

    Keywords: Diatome, Plankton, Percoll

    References

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