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    E-ISSN: 2149-4533 ISSN: 1300-865X
    Spermatozoa Isolation and ABO(H) Group Typing with Proteinase K on Semen Contaminated Vaginal Material
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    Research Article
    VOLUME: 2 ISSUE: 3
    P: 118-123
    December 1997

    Spermatozoa Isolation and ABO(H) Group Typing with Proteinase K on Semen Contaminated Vaginal Material

    The Bulletin of Legal Medicine 1997;2(3):118-123
    DOI: 10.17986/blm.199723238
    Sema DEMİRÇİN KARAGÖZ 1
    Beyhan EGE 2
    1. Uzm. Dr., Antalya Devlet Hastanesi, Antalya İl Sağlık Müdürlüğü
    2. Prof. Dr.,Ege Üniversitesi Tıp Fakültesi Adli Tıp Anabilim Dalı, Adli Tıp Kurumu İzmir Grup Başkanlığı
    No information available.
    No information available
    Received Date: 03.02.1997
    Accepted Date: 22.05.1998
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    ABSTRACT

    Physical and gynecological examinations and detection of spermatozoa in vaginal material are the procedures used to prove the sexual crimes as rape, sexual assault, sodomy and adultary cases. Besides these examinations, assessment of genetic markers in the vaginal material is necessary to identify the offender in cases of sexual assault with unknown perpetrator. In this study, the applicability of an absorbtion elution technique with proteinase K treatment is investigated to increase the reliability of ABO(H) group typing on semen contaminated vaginal swabs. It was seen that after Proteinase K treatment only spermatozoa remain in vaginal material and blood group obtained in conclusive reflects only the antigenic structure of spermatooza. It was calculated that ratio of obtaining a true result was 82.67 % with Proteinase K treated samples and 86 % with nontreated samples.

    Keywords: ABO(H) typing, vaginal material, sexual assault, proteinase K, spermatozoa

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